| TÃtulo : |
ENZYMES: Catalysis, Kinetics and Mechanisms |
| Tipo de documento: |
documento electrónico |
| Autores: |
Punekar, N.S, |
| Mención de edición: |
1 ed. |
| Editorial: |
Singapore [Malasia] : Springer |
| Fecha de publicación: |
2018 |
| Número de páginas: |
XXIII, 562 p. 263 ilustraciones, 76 ilustraciones en color. |
| ISBN/ISSN/DL: |
978-981-1307850-- |
| Nota general: |
Libro disponible en la plataforma SpringerLink. Descarga y lectura en formatos PDF, HTML y ePub. Descarga completa o por capítulos. |
| Idioma : |
Inglés (eng) |
| Palabras clave: |
EnzimologÃa ProteÃnas BiotecnologÃa MicrobiologÃa industrial BioquÃmica de proteÃnas |
| Clasificación: |
572.7 |
| Resumen: |
Este libro de texto de enzimologÃa para estudiantes de posgrado y de grado avanzado cubre los programas de estudio de la mayorÃa de las universidades donde se enseña regularmente esta materia. Se centra en la sincronÃa entre las dos amplias facetas mecanicistas de la enzimologÃa: la quÃmica y la cinética, y también destaca la sinergia entre la estructura y el mecanismo de las enzimas. Diseñado para el autoaprendizaje, explica cómo diseñar experimentos enzimáticos y, posteriormente, analizar los datos recopilados. El libro está dividido en cinco secciones principales: 1] Introducción a las enzimas, 2] Aspectos prácticos, 3] Mecanismos cinéticos, 4] Mecanismos quÃmicos y 5] Fronteras de la enzimologÃa. Los conceptos individuales se tratan como capÃtulos independientes; los lectores pueden explorar cualquier concepto individual con una mÃnima referencia cruzada con el resto del libro. Además, los enfoques complejos que requieren técnicas especializadas y experimentación involucrada (más allá del alcance de un laboratorio promedio) se cubren en teorÃa con referencias adecuadas para guiar a los lectores. El libro proporciona a los estudiantes, investigadores y académicos en el amplio campo de la biologÃa un sólido conocimiento teórico y práctico de las enzimas. También está dirigido a aquellos que no cuentan con un enzimólogo en ejercicio que les enseñe la materia. |
| Nota de contenido: |
Part I: Enzyme catalysis - a perspective -- Enzymes -Their place in Biology -- Enzymes - Historical aspects -- Exploiting enzymes – Technology -- On enzyme nomenclature and classification -- Hallmarks of an enzyme catalyst -- Origins of enzyme catalytic power -- Origins of enzyme catalytic power -- Which enzyme uses what trick? Some remarks -- Structure and catalysis: Conformational flexibility and protein motion -- Part II: Enzyme kinetic practice and measurements -- Chemical kinetics – Fundamentals -- Concepts of equilibrium and steady-state -- ES complex and pre-steady-state kinetics -- Principles of enzyme assays -- Good kinetic practices -- Quantification of catalysis and measures of enzyme purity -- Henri-Michaelis-Menten equation -- More complex rate expressions -- Enzyme kinetic data - Collection and analysis -- Part III: Elucidation of Kinetic mechanisms -- Approaches to kinetic mechanism – Overview -- Analysis of initial velocity patterns -- Enzyme inhibition analyses -- Irreversible inhibitions -- Reversible inhibitions -- Alternate substrate (product) interactions -- pH Studies with enzymes -- Isotopes in enzymology -- Isotope exchanges at equilibrium -- Isotope effects in enzymology -- From kinetic data to mechanism and back -- Part IV: Chemical mechanisms and catalysis -- Chemical reactivity and molecular interactions -- Acid-base chemistry and catalysis -- Nucleophilic catalysis and covalent reaction intermediates -- Phosphoryl group chemistry and importance of ATP -- Enzymatic oxidation-reduction reactions -- Carboxylations and decarboxylations -- Electrophilic catalysis and amino acid transformations -- Integrating kinetic and chemical mechanisms – A synthesis -- Part V: Frontiers in enzymology -- Regulation of enzyme activity -- In vitro versus in vivo - Concept and consequences -- Future of enzymology – An appraisal -- Closure - Whither Enzymology. |
| Tipo de medio : |
Computadora |
| Summary : |
This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to design enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject. |
| Enlace de acceso : |
https://link-springer-com.biblioproxy.umanizales.edu.co/referencework/10.1007/97 [...] |
ENZYMES: Catalysis, Kinetics and Mechanisms [documento electrónico] / Punekar, N.S, . - 1 ed. . - Singapore [Malasia] : Springer, 2018 . - XXIII, 562 p. 263 ilustraciones, 76 ilustraciones en color. ISBN : 978-981-1307850-- Libro disponible en la plataforma SpringerLink. Descarga y lectura en formatos PDF, HTML y ePub. Descarga completa o por capítulos. Idioma : Inglés ( eng)
| Palabras clave: |
EnzimologÃa ProteÃnas BiotecnologÃa MicrobiologÃa industrial BioquÃmica de proteÃnas |
| Clasificación: |
572.7 |
| Resumen: |
Este libro de texto de enzimologÃa para estudiantes de posgrado y de grado avanzado cubre los programas de estudio de la mayorÃa de las universidades donde se enseña regularmente esta materia. Se centra en la sincronÃa entre las dos amplias facetas mecanicistas de la enzimologÃa: la quÃmica y la cinética, y también destaca la sinergia entre la estructura y el mecanismo de las enzimas. Diseñado para el autoaprendizaje, explica cómo diseñar experimentos enzimáticos y, posteriormente, analizar los datos recopilados. El libro está dividido en cinco secciones principales: 1] Introducción a las enzimas, 2] Aspectos prácticos, 3] Mecanismos cinéticos, 4] Mecanismos quÃmicos y 5] Fronteras de la enzimologÃa. Los conceptos individuales se tratan como capÃtulos independientes; los lectores pueden explorar cualquier concepto individual con una mÃnima referencia cruzada con el resto del libro. Además, los enfoques complejos que requieren técnicas especializadas y experimentación involucrada (más allá del alcance de un laboratorio promedio) se cubren en teorÃa con referencias adecuadas para guiar a los lectores. El libro proporciona a los estudiantes, investigadores y académicos en el amplio campo de la biologÃa un sólido conocimiento teórico y práctico de las enzimas. También está dirigido a aquellos que no cuentan con un enzimólogo en ejercicio que les enseñe la materia. |
| Nota de contenido: |
Part I: Enzyme catalysis - a perspective -- Enzymes -Their place in Biology -- Enzymes - Historical aspects -- Exploiting enzymes – Technology -- On enzyme nomenclature and classification -- Hallmarks of an enzyme catalyst -- Origins of enzyme catalytic power -- Origins of enzyme catalytic power -- Which enzyme uses what trick? Some remarks -- Structure and catalysis: Conformational flexibility and protein motion -- Part II: Enzyme kinetic practice and measurements -- Chemical kinetics – Fundamentals -- Concepts of equilibrium and steady-state -- ES complex and pre-steady-state kinetics -- Principles of enzyme assays -- Good kinetic practices -- Quantification of catalysis and measures of enzyme purity -- Henri-Michaelis-Menten equation -- More complex rate expressions -- Enzyme kinetic data - Collection and analysis -- Part III: Elucidation of Kinetic mechanisms -- Approaches to kinetic mechanism – Overview -- Analysis of initial velocity patterns -- Enzyme inhibition analyses -- Irreversible inhibitions -- Reversible inhibitions -- Alternate substrate (product) interactions -- pH Studies with enzymes -- Isotopes in enzymology -- Isotope exchanges at equilibrium -- Isotope effects in enzymology -- From kinetic data to mechanism and back -- Part IV: Chemical mechanisms and catalysis -- Chemical reactivity and molecular interactions -- Acid-base chemistry and catalysis -- Nucleophilic catalysis and covalent reaction intermediates -- Phosphoryl group chemistry and importance of ATP -- Enzymatic oxidation-reduction reactions -- Carboxylations and decarboxylations -- Electrophilic catalysis and amino acid transformations -- Integrating kinetic and chemical mechanisms – A synthesis -- Part V: Frontiers in enzymology -- Regulation of enzyme activity -- In vitro versus in vivo - Concept and consequences -- Future of enzymology – An appraisal -- Closure - Whither Enzymology. |
| Tipo de medio : |
Computadora |
| Summary : |
This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to design enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject. |
| Enlace de acceso : |
https://link-springer-com.biblioproxy.umanizales.edu.co/referencework/10.1007/97 [...] |
|  |
Crear una solicitud de compra Refinar búsqueda
